Playing in Traffic: Novel Signaling and Trafficking Mechanisms in the Kidney
Professor Michael Caplan
C.N.H. Long Professor and Chair of
the Department of Cellular and Molecular Physiology,
Yale University School of Medicine
Tuesday 13 November 2012, 5.30pm-6.30pm
JZ Young Lecture Theatre
UCL Medical Sciences and Anatomy Building
Gower Street, London. WC1E 6BT
This is a public event, and is open to all, thasnk to the generous support of SHM Productions. This lecture will be of particular interest not only to medical students, medical researchers and other medical professionals but also to the informed general public.
The lecture will be followed by a reception for Professor Sessa, held in the North Cloister, UCL Wilkins Building, to which all ticket holders are cordially invited.
Posters for this event, suitable for local printing, may be downloaded as follows:
Black and white:
Abstract: The physiological properties of epithelial cells are determined largely by the ion transport proteins that occupy their apical and basolateral plasma membrane domains. Generation and maintenance of epithelial polarity requires cells to possess sorting machinery that discriminates among newly synthesized membrane proteins and sends them to their appropriate subcellular destinations. To follow the post-synthetic fate of newly synthesized epithelial membrane proteins, we have adapted a technique that permits direct observation of temporally-defined cohorts of proteins via the combination of fluorescence microscopy with pulse-chase labeling protocols. This technique also allows us to purify temporally-defined cohorts of proteins of interest and thus to begin to define their “temporal interactomes”.
Perturbations in epithelial morphogenesis are associated with a number of human genetic diseases, the most common of which is Autosomal Dominant Polycystic Kidney Disease (ADPKD). Affecting ~1:1,000 individuals, ADPKD is associated with the replacement of renal nephrons with fluid-filled cysts that ultimately compromise renal function. Although the genes responsible for ADPKD are known, their functions remain mysterious. We find that one of the proteins encoded by an ADPKD gene undergoes a cleavage that releases a fragment that enters the nucleus and modulates transcription. We have begun to elucidate the signaling pathways involved in this process and have identified new potential therapeutic targets.
Finally, we find that the signal transduction machinery present in the cilia of olfactory neurons is also present in renal epithelial cells. Our data suggest that olfactory receptors participate in regulating renal function in response to chemosensory cues.
When & Where
Yale UCL Collaborative (Biomedicine)
UCL and UCL Partners have entered into a unique collaboration with Yale University, Yale School of Medicine, Yale-New Haven Hospital, called the Yale UCL Collaborative. More ..