Advances in Biophysical Methods for Protein Characterisation Conference
Protein dynamics, structure, and stability are essential factors for biological activity, the proper function of protein-based biotechnology products, as well as their efficacy and safety as pharmaceuticals. Experimental characterisation of each of these factors requires multiple biophysical techniques, spanning from light / x-ray / neutron scattering to spectroscopy, calorimetry, electrophoresis, microscopy, densimetry, rheology, and surface characterization. This conference focuses on advances in biophysical characterization of proteins in solution and the solid state, as well as proteins at bulk interfaces, from the perspective of fundamental and applied biotechnology, including biopharmaceuticals. This includes novel uses of existing methods, as well as development of new methods or adaptions of methods from other fields.
Keynote speakers: Prof. Roland Winter (TU Dortmund) and Dr. Christian Rischel (Novo Nordisk)
Full Conference Programme:
Monday, 3rd October 2016
18:00-20:00 Registration desk
19:00 Welcome drinks
Tuesday, 4th October 2016
08:30-10:30 Registration desk
8:30 – 8:40 Opening remarks, Dan Bracewell, University College London
8:40-9:30 Keynote presentation, Christian Rischel, Novo Nordisk, Understanding Protein Stability to Make New Drugs
Session 1. Protein folding, dynamics, and higher-order structure
9:30-10:00. Antonio Cupane, University of Palermo, Dynamics of water in biophysical systems: from water confined in silica xerogels and in paper cellulose matrices to protein hydration water
10:00-10:30 Claudia Capitini, University of Florence, Structural determination of HypF-N oligomersby Förster Resonance Energy Transfer (FRET)
10:30-11:00 Mark Telling, Rutherford Appleton Laboratory, Unravelling protein dynamics: what can high energy resolution neutron spectroscopy tell us?
11:15-11:45 Annalisa Pastore, King’s College London, The role of electrostatic frustration in cold denaturation
11:45-12:15 Valentino Bianco, University of Vienna, The role of water in the selection of stable proteins at ambient and extremethermodynamic conditions
12:15-12:45 Seyyed Abolghasem Ghadami, University of Florence, Analysis of Transthyretin partially folded states by FRET
12:45-1:15 Dimitri E. Khoshtariya, Tbilisi State University, Combined studies of conformational and mechanistic issues for redox-active proteins through the extended method of interfacial voltammetry
13:15 – 14:15 Lunch
Session 2. Protein (mis)folding and aggregation
14:15-14:45 Samuele Raccosta, National Research Council of Italy, Institute of Biophysics, Serpin conformational diseases: From molecular studies to therapeutic intervention
14:45-15:15 James A. Irving, University College London, Modulating stability and structural change in α1-antitrypsin
15:15-15:45 Carlotta Marasini, University of Copenhagen, Sucrose as a crowding agent: influence on insulin fibril structure and kinetics
15:45-16:15 Francesco Bemporad, University of Florence, Mixing folding and misfolding kinetics with spectroscopic investigations to shed light on the pathways that lead to amyloid formation
16:30-17:00 Birgit Strödel, University of Düsseldorf, Advanced all-atoms simulations of amyloid aggregation
17:00-17:30 Benedetta Mannini, University of Cambridge, A proteomic approach to study the interactome of misfolded oligomers
17:30-18:00 Sarah Hedberg, Imperial College London, Mapping the Aggregation Behaviour of Biopharmaceuticals: A New Approach
18:00-18:30 Poster Presenters – Oral Abstracts
18:30-19:00 Reception and Posters
Wednesday, 5th October 2016
9:00-9:50 Keynote presentation, Roland Winter, Technische Universität Dortmund, Exploring the Conformational Space and Dynamics of Biomolecular Systems Using Pressure Perturbation
Session 3. Proteins in the solid state and crowded environments
9:50-10:20 Michael J. Pikal, University of Connecticut, Pharmaceutical Stability of Proteins in the Solid State: Critical Factors in Formulation and Processing of Freeze Dried Proteins
10:20-10:50 Eric Munson, University of Kentucky, Using Solid-State NMR Spectroscopy as a Predictor of Lyophilized Protein Formulation Stability
10:50-11:20 Viviana Cristiglio, Institut Laue Langevin, Monitoring of protein interactions in frozen and freeze-dried solution states using small angle scattering techniques
11:35-12:05 Miguel A. Rodrigues, Instituto Superior Técnico, University of Lisbon, Clarifying the thermodynamic influence of cold and cryoprotectants with isochoric protein aggregation studies below the freezing temperature
12:05-12:35 S. Longeville, Laboratoire Léon Brillouin, Crowding effect of TMAO on the structural stability of myoglobin during thermal unfolding
12:35-1:05 Loredana Randazzo, National Research Council of Italy, Institute of Biophysics, Diffusion, stability and activity of neuroserpin in crowded media
Afternoon recess (with tour(s) of Palermo available)
19:00-19:30 Reception and Posters
19:30 Conference Dinner
Thursday, 6th October 2016
Session 4. Protein-protein and protein-surface interactions
9:00-9:30 Joel Kaar, University of Colorado Boulder, Connecting Protein Structure and Interfacial Dynamics with Single-Molecule Methods
9:30-10:00 Alice Mazzer, University College London, Measuring protein structures at chromatographic interfaces using neutron reflection
10:00-10:30 Luke Clifton, Rutherford Appleton Laboratory, The Binding of Antimicrobial Agents to Model Gram Negative Bacterial Surfaces
10:30-11:00 Max Hebditch, University of Manchester, Sequence and structural properties influencing FAb solubility in solution
11:15-11:45 Jayne Lawrence, King’s College London, Antimicrobial Peptides: Interactions with Model Membranes?
11:45-12:15 Paulo Arosio, University of Cambridge, Microfluidic Diffusion Analysis of the Sizes, Interactions and Viscosities of Proteins under Native Solution Conditions
12:15-12:45 Robin Curtis, University of Manchester, A combined electrophoretic and light scattering approach to elucidate the molecular origin of reverse Hofmeister series effects
12:45-1:15 Chris Roberts, University of Delaware, Quantifying and Predicting Protein-Protein Interactions from Low to High Concentrations
13:15 Concluding Group Discussion and Remarks
Call for Abstracts:
Abstracts are requested for oral presentations and poster presentations focused on experimental and theoretical studies of protein dynamics, structure, and stability as they pertain to the proper function of protein-based biotechnology products. Specific topic areas include: higher order structural characterization, protein (mis)folding and aggregation, protein adsorption to bulk interfaces, protein-protein interactions, solid-state proteins, and advanced characterization techniques across any of these areas.
Abstracts are limited to 250 words, and should be submitted via email as a Word document (one file per submission) to: Maria Papachristodoulou .
Due date: Has been extented to 17 of June 2016 for oral presentations; 29 June 2016 for poster presentations.
Presenters will be notified by 1 July 2016 (oral presentations) and 5 August 2016 (poster presentations) regarding accepted abstracts, to provide sufficient notice for travel planning purposes.
Questions regarding abstracts and deadlines should be addressed to Maria Papachristodoulou .
Students can apply for reduced registration fee.For more information please email at <email@example.com>.
Early bird tickets will no longer be available for sale after the 1/9/2016 at 23:30
Regular tickets will not longer be available for sale after the 4/10/2016 at 23:30
PhD students have a 50% discount on the ticket price
The above dates are also true for the student tickets
5 coffee breaks
2 light lunches
1 Welcome Cocktail
Day 2 evening reception
October is a high season for business events in Palermo so please try and book well in advance.
When booking please specify in the request box that you are attending the Advances in Biophysical Methods for Protein Characterisation Conference.
The hotel links are:
Grand Hotel Piazza Borsa: http://www.piazzaborsa.it/index.php/it/
Hotel Porta Felice: www.hotelportafelice.it
Hotel Palazzo Sitano: www.hotelpalazzositano.it
Quintocanto Hotel: www.quintocantohotel.com
Hotel Ambasciatori: www.ambasciatorihotelpalermo.com