QUB Fluorescence Course by Dr Richard Thompson

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The Wellcome-Wolfson Institute for Experimental Medicine (CEM)

97 Lisburn Road

Belfast

BT9 7BL

United Kingdom

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The course will concentrate on making your future work using fluorescence and confocal microscopy significantly more successful.

About this Event

QUB Fluorescence Course by Dr Richard Thompson

University of Baltimore, School of Medicine

15th of October 1.00-4.30 pm

17th of October 1.00-4.30 pm

Basement Seminar Room, WWIEM (CEM)

Two years ago Dr Thompson led a 2 half day course on basic and advanced fluorescence imaging with great success. Over 50 participated in this and the3 feedback was that they all took away a lot of knowledge they did not even think they needed. This course will concentrate on making your future work using fluorescence and confocal microscopy significantly more successful. We have amazing facilities at QUB and therefore I would like to encourage all of you to attend. The lecture series will be held at the Wellcome Wolfson Institute for Experimental Medicine and is supported by the School of Medicine and Dentistry and Prof Alan Stitt.

Organiser: Dr Imre Lengyel, QUB

i.lengyel@qub.ac.uk

About Dr Thompson:

I received a B.A. in Biology from Northwestern University and the Ph.D. in Biochemistry from the University of Illinois at Urbana-Champaign with Thomas Baldwin on the primary structure of bacterial luciferase. I did postdoctoral training with Joseph Lakowicz at the University of Maryland, Baltimore (UMB), on the effects of high pressure on biomolecules, then was awarded a National Research Council Associateship at the U.S. Naval Research Laboratory in Washington, DC. I continued work on fluorescence-based fiber optic sensors at NRL as Supervisory Research Chemist, and received a U.S. Navy Special Act Award for Operation Desert Storm.

15th of October 1.00-4.30 pm

I. Fundamentals of Fluorescence

Basic physics of fluorescence, almost without annoying theory

Spectra and the Golden Rules

Properties of Fluorescent Molecules and What you can learn

Spectra

Anisotropy (Polarization)

Lifetime (an appetizer)

COFFEE AND BISCUITS

II. Fundamentals of Instrumentation and Probes

Overall Design Features of Fluorometers

Light Sources:

Lamps

LEDs

Lasers: CW and pulsed

Monochromators and Filters

Cuvettes and Sample Compartments

Detectors

Single pixel: PMTs, photodiodes

Imaging detectors (CCD and CMOS)

Electronics: Direct amplification vs photon counting

17th of October 1.00-4.30 pm

III. Time-Resolved Fluorescence

Concept of Fluorescence Lifetime

Time-Domain Measurements

Frequency-Domain Measurements

Fluorescence Lifetime-Based Sensing

COFFEE AND BISCUITS

IV. Microscopy and FLIM

Basics of Microscopy and Fluorescence Microscopy

Fluorescence Lifetime Microscopy (FLIM)

Two-Photon, Superresolution, Single Molecule, and other Costly Microscopies

Date and Time

Location

The Wellcome-Wolfson Institute for Experimental Medicine (CEM)

97 Lisburn Road

Belfast

BT9 7BL

United Kingdom

View Map

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