UPLC User Training by Waters x
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UPLC User Training by Waters x

UPLC User Training by Waters x

Sales Have Ended

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Booking for this session is now closed.

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Lonza Slough

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Sales Have Ended

Registrations are closed
Booking for this session is now closed.
Event description

Description

For Lonza Employees Only

Acquity UPLC H-Class Bio for lab practitioners

Instruction for the ACQUITY UPLC H-Class Bio is a two day course designed to offer a solid understanding of ACQUITY UPLC H-Class Chemistry, Instrumentation and Software. Instrument overview, chromatographic concepts as well as Method Transfer and Development will be taught.

Topics Covered

UPLC theory & chemistry

  • UPLC principle & theory
  • Resolution & peak capacity
  • UPLC column chemistries
  • Column design
  • System bandspreading

ACQUITY hardware

  • Solvent manager
  • Degasser
  • Sample manager
  • Column manager
  • eCord technology
  • Detectors

ACQUITY console software

  • Overview of console software
  • Module functions and diagnostics
  • Interactive displays
  • Plots and logs
  • Preparing for analysis
  • Characterizing needle and loop volume
  • Instrument method parameters

Method transfer, HPLC to UPLC

  • Method transfer considerations
  • Transfer of isocratic methods
  • Transfer of gradient methods
  • System delay volume
  • Using the ACQUITY column calculator

Troubleshooting tips and tricks

  • Mobile phase preparation
  • Controlling bacterial growth
  • Column maintenance
  • Tips and tricks for the solvent and sample manager
  • Optimal detector settings
  • Troubleshooting peak shape problems and carry over

Hands-on exercises

Who Should Attend

The course is designed for users who currently use the ACQUITY UPLC H-Class Bio System. For the user who is involved in Instrument Operation and for those whose responsibilities include Method Transfer and/or Method Development

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Lonza Slough

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