Advanced Flow Cytometry: -1Day

Building Block: Advanced Flow Cytometry

This interactive course builds on the Introductory course and goes into greater details on how a cytometer works, best practices for running cytometry experiments, experimental design, panel design, compensation and data spreading and presentation of data.

As cytometers become capable of measuring an increasing number of fluorochromes, experimental design becomes more complicated. It requires knowledge of the fluorochrome, the biology (antigen density, co-expression) and the sample preparation as well as as a greater understanding about the cytometer. In this one-day course we will look at the factors that need to be taken into account to build multicolour panels. We will do several interactive panel designs where we will use this knowledge to design panels of 6-10 and discuss panels with larger numbers of fluorochromes.

The in person course includes refreshments, including lunch, morning and afternoon tea and coffee.