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Bristol BioSAXS Workshop 2016

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School of Biochemistry

Medical Sciences Building

Medical Sciences, University Walk

Bristol, United Kingdom

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We invite you to join the 2016 Bristol BioSAXS workshop, which will run over two days on the 17th-18th November 2016 in the School of Biochemistry at Bristol University. The workshop will be led by Dr Robert Rambo, the Principal Beamline Scientist for B21, a dedicated solution state Small Angle X-ray Scattering (SAXS) beamline at Diamond Light Source, UK.

This workshop is a great opportunity for those interested in using SAXS for biological structure elucidation in solution. No prior SAXS or synchrotron experience is required but the workshop will also be useful for more experienced users. Attendees will be invited to submit samples for measurement on the B21 beamline prior to the workshop. Day one will cover theoretical and practical aspects of SAXS measurement, with day two focusing on analysis of your own data from the B21 beamline using the Sctter, a bioSAXS analysis application developed by Dr Rambo.

 

If you are interested in attending the workshop, please RSVP using the Eventbrite ticket system. Samples must be ready before the 1st of October, and may submitted by post to B21, or in person at the University of Bristol.

 

Please contact Kristian Le Vay (kristian.levay@bristol.ac.uk)  with any questions and for details regarding sample requirements and submission. For more information on solution structure elucidation using SAXS, please see http://www.bioisis.net/tutorial

 

Biography

 

Dr Rambo initially trained as an RNA crystallographer in the laboratory of Jennifer A. Doudna at Yale University, where he helped determine the structure of the bacterial signal recognition particle (SRP) and a structural role for potassium ions in the P4-P6 RNA domain (the organizational centre for the group I intron). After graduating, Robert worked briefly as a web-application programmer before starting his post-doctoral training at the Advanced Light Source with the SIBYLS beamline, a dual SAXS and MX development beamline headed by John A. Tainer of the Scripps Research Institute. Here, Robert helped establish standard purification methods for RNA SAXS experiments and developed new methods for analysing SAXS data, including the volume-of-correlation and chi-free mass determination. Robert’s expertise is in applying new mathematical methods (convex optimization and semi-definite programming) to SAXS experiments, making B21 a competitive centre for methods development. Dr Rambo actively develops SAXS software including of Sctter, a java-based program for bioSAXS. He is the creator of BioIsis.net, a solution SAXS database. ­­­­

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School of Biochemistry

Medical Sciences Building

Medical Sciences, University Walk

Bristol, United Kingdom

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