DEPArray™ Digital Sorting to Resolve Heterogeneity on Low Input and Low Cellularity FFPE Tumors and Circulating Tumor Cells

The Oxford Genomics Centre is one of the largest genomics facilities in the UK. We specialize in the latest in sequencing and array technology from Illumina, with six HiSeqs, two MiSeqs and an iScan system. Our sequencing applications include whole-genome sequencing, targeted sequencing, RNAseq, ChIPseq and methylation analysis. We also undertake genotyping and gene expression assays with a new Affymetrix Gene Titan system, and focused genotyping using the Sequenom platform. We have recently installed a Fluidigm C1 system and a Fluidigm BioMark HD to enable single-cell capture and profiling experiments. With dedicated automation and QC pipelines we support a wide range of experimental protocols.

Oxford Genomics Centre, Wellcome Trust Centre for Human Genetics, University of Oxford

DEPArray™ is the only sorting technology capable of isolating, through a semiconductor controlled microfluidic chip, 100% pure single or pooled cells. Single Circulating Tumor Cells can be isolated from enriched blood, pools of pure Tumor, EMT, TILs and Stromal cells can be isolated  from disaggregated FFPE tissue blocks, as well as live cells can be isolated from fresh tumor tissue.
NGS Results show how interpretation of underlying genetic alterations becomes far clearer on samples transformed into collections of pure or single cells, not only improving the detection of Somatic Mutations, but enabling highly informative Copy Number Aberration analysis on the pure cell populations.
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